From the Gallery of Movable Instruments, one enters the Meridian Room, which was part of the original architectural layout of the Specola and was initially designed to house a Ramsden Transit Instrument, now lost. Around 1858, the room underwent a major transformation. The project, carried out by court architect Nicolò Puglia, involved expanding the space and reinforcing the floor to make it sturdy enough to support the heavy Billiemi stone pillars that would hold the Meridian Circle by Pistor & Martins - a new telescope from Berlin.
Architect Giovan Battista Filippo Basile also contributed to the renewed appearance of the room, designing the neo-Gothic wooden decorations that adorn the new Meridian Room. The ceiling features a slit aligned with the local meridian, allowing the telescope to observe stars as they cross the meridian. By recording the precise moment of their transit using clocks and chronographs - still preserved in the room - astronomers could determine their astronomical coordinates.